Tuesday, May 31, 2016

Cray fish dissection

Crayfish are freshwater crustaceans that live in streams, rivers, and lakes. While they can be found practically everywhere in the world with these conditions, crayfish are most abundant in the United States/ North America. Crayfish eat decaying plants, dead fish or insects, and living plants. Like fish, crayfish have gills that they breathe through in order to obtain oxygen that is then dissolved into their bloodstream. One fun fact is that there are many different unique colors of crayfish that can be found such as bright blue, green, and orange.







Internal:
Green Gland: Digestive enzyme that produced digestive glands
Flexor Muscles: Help move the body of the crayfish and maintain coordination in water
Anterior Gastric Muscle: Help the stomach in digestion

External:
Cheliped: Used for defense and to capture prey
Walking legs: help the crayfish move from one location to another
Swimmerets: Abdominal appendage that help move water
Antennae: Senses taste and touch
Incision guide






Video link

Clam dissection

Clams are a part of the Mollusca phylum and the Animalia kingdom. Different species can be found in both salt and freshwater environments. They can often be found buried in the sand or in the mud. Clams eat plankton in order to gain energy and survive. They are able to eat them by pumping water containing the plankton through their gills. Clams breathe through their gills just like fish do. One fun fact about clams is that they do not have eyes, ears, or a nose!









Internal:
Foot: Used to dig down into the sand and draw nourishment.
Gonad: Produce gametes
Gills: Help to exchange gases and provide air.
Posterior Adductor: Pulls foot back in shell

External:
Umbo: Oldest part of clam shell, it’s a bump on the shell
Mantle: Tissue that lines valves
Growth Ring: Can help determine age of clam

Incision guide

Video link 

Earthworm dissection

Earthworms are a part of the Annelida phylum, and the Lumbricidae family. Earthworms live in the soil, and because soil is so abundant around the world, they can be found pretty much anywhere. Earthworms get their nutrition from decaying things, animal feces, or decomposing animals in the soil. Worms do not breathe through lungs, and instead it absorbs oxygen from its skin. Then, oxygen is dissolved into the bloodstream so that the worm can function and survive.
One fun fact about them is that earthworms are both female and male because one earthworm produces sperm and eggs.








Internal:
1.Crop: Food is stored here for the earthworm
2. Gizzard: Food is crushed here after moving from the crop using stones.
3.Pseudohearts: Keep the blood flowing throughout the body.
4. Esophagus: Connection to crop helps move food for digestion
External:
1. Anus: Where the digested waste comes out from
2. Mouth: Entrance for food
3. Setae: Help anchor worm when moving in soil
Incision guide















Video link


https://m.youtube.com/watch?v=jEPddCPuHXQ





Perch dissection


        Perch is a freshwater fish that can be found in the lakes and rivers of North America. Their diet consists of a variety of organisms such as snails, crayfish, mussels, leeches, insects, worms, and even fish eggs. Perch breathe by taking water in through their mouths, and then passing it through its gills. When this occurs, oxygen is able to dissolve into the bloodstream. Something interesting about perch is that they are not very good swimmers because they are not able to speed up quickly and easily. 


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External: 
Eye: Helps the perch to see in the water
Pectoral Fins: Allows fish to tune its movement and keeps it from moving to the side. 
Nares: Nostrils help remove moisture from exhalation
Spinal Dorsal Fin: Stabilize during sudden turns
Internal:
Kidney: Helps regulate water and salt balance within the fish
Gills: Exchange gases and transfer ions.
Spleen: Reservoir for blood within the perch
Stomach: Helps with mechanical and chemical digestion.

Incision guide












Video link
https://m.youtube.com/watch?v=FKG7F6s4TCk




Frog dissection

Frog

Frogs are a part of the Amphibia family, so they live on land but reproduce in the water. They can be found on all of the continents besides Antarctica, because the living conditions are too harsh for them to survive. There are 90 species of frogs in the US today. Frogs eat insects such as moths, flies, dragonflies, and mosquitoes. It's food is swallowed through the esophagus and then is passed through the digestive tract and the digestive glands. From there it goes through the stomach and is passed through the small intestine where it can digest its food. Frogs breathe through their nostrils and larynx, which are a part of the respiratory system. They have 2 lungs which have capillaries that allow materials to pass in and out of the body. One fun fact about frogs is that they are able to jump further than 20 times the length of their body.
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Internal:
1.Lungs: Lungs help exchange gasses and filter the air to allow the frog to breathe on land.
2.Stomach: Helps to digest food (mechanical and chemical digestion).
3.Intestines: Final digestion and absorption of nutrients
4.Gallbladder: Produces bile
5. Vomer teeth: Help the frog chew
External:
1.Eyes: help the frog to see above the water and in the water
2. Nictitating membrane: helps to moisten the eye while allowing the frog to see.
3.Tympanum: Transmits sound into inner ear
Incision guide




Link to dissection video 


 





Friday, May 13, 2016

Artificial Selection Lab

Artificial Selection Lab
The purpose of the lab was to artificially select the traits we wanted to express in the second generation of the fast plants seeds. We wanted to see if we could pick and choose which plants it breed together to come up with a specific phenotype.


    Evolution occurs when characteristics of a population evolve over a long period of time. In natural selection, genes of a population that are favored will be passed to offspring, continuing the presence of the trait. Traits that are not beneficial to the organism, and possibly even harm them, are not passed on to the next generation and die out. This could be due to change in the environment, natural selection of genes, or another change in traits. Artificial selection is the opposite of natural selection, because the environment is not determining the traits. It is the ability of humans to alter the way in which evolution works by controlling which individuals will reproduce., therefore controlling the results of the cross. This process does not occur naturally in the environment, and can only be done by human control. By doing this, we can attempt to control or predict the traits of their offspring. Plants can be artificially selected by choosing which breeds of flowers will reproduce with another. Another example of artificial selection are the different breeds of dogs that we have. Humans have picked and altered the traits of wolves, choosing which traits offspring should have by selecting which specimen will reproduce. In this lab, we attempted to artificially select which traits the offspring of the parental generation would have. By choosing which traits we want for the offspring, we determine which parent generations to cross.


Procedures: We got a cup of dirt to put in our pot and labeled which type of plant we had to keep track of growth and so we could pick which plant to breed. We cut a wicking cord to feed it through the bottom so it could distribute water to the plant.


Next, we planted the F1 generation of seeds and monitored growth


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Finally, we monitored growth over a long period of time. For a while we were basically just watering dirt, but eventually our plants began to grow a little bit.


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Next, we went on a three day weekend and came back to all dead plants that looked something like this due to a lack of water. Possibly due to the failed wicking cord.


Lastly, after using the cheat seeds we began to see actual flowers. I think that due to us being on top of our watering after having dead plants in the reason that we saw so much growth.
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We planted F1 seeds. We planted three purple stem hairy seeds, three non purple stem yellow green leaf, and three F1 non purple hairless( this one is short). They began to grow the first couple days and weeks. After about two weeks or so they died. This could be due to lack of water. On Friday they were alive after a three day weekend they were no longer living, fear not they provided nutrients to the soil. We used the cheat seeds for the F2 generation. We did not actually artificial select the traits we wanted to see expressed in the F2 generation because all of our F1 plants died. From the F2 seeds we should have seen 9 purple stemmed green leafed, 3 purple stemmed yellow leaves, 3 green stemmed green leaves, 1 green stemmed yellow leaves. We saw one purple stemmed yellow leaved flower, one green stemmed green leaved flower, and one green stemmed yellow leaved flower. The difference in observed and expected phenotype counts could be due to some of the plants not sprouting. The plants that did sprout could have blocked other plants from sprouting. The seeds on the top of the soil could have not sprouted because they could have been displaced after we watered the plants. It could also be due to potentially defective seeds that may be old or that didn’t sprout properly because they were under light. The seeds that were too deep in the soil didn't sprout because they didn't get enough sunlight. The wicking cord could have unevenly supplied water for some plants. Human error of neglect also played a role especially in the F1 generation. The lack of water for a few days caused the F1 generation to die.  




In conclusion, this lab was not completely successful. Due to human error the plants in the F1 generation died and thus we were not able to breed the plants to come up with our own expressed traits. If done correctly, we should have seen some tall plants some plants with purple stems and some short plants and some with green stems. These would have resulted in a 9:3:3:1 ratio but some of the plants didn't grow at all. This could also be a result of defective seeds, causing the plant to not grade at all. Essentially, we should have seen a variety of phenotypes fromt the plants which we did not due human error, possibly defective seeds, or lack of water.